Ego ole ka a ga-etinye n'ime karama ahụ n'ime incubator na-ama jijiji?
A na-emepụta eriri Erlenmeyer maka imepụta mkpụrụ ndụ nkwụsịtụ gụnyere mkpụrụ ndụ HEK 293 na CHO n'ime sel ndị na-adịghị ike.onye na-ama jijiji incubator. Ihe ndị a nwere ala dị larịị na mkpuchi ikuku, nke na-eme ka ha dị mma maka mmepe obere usoro, mmụba, na ịkụ ihe n'ọtụtụ ọkwa. Mkpuchi ikuku a na-etinye na-ejikọta akpụkpọ ahụ hydrophobic nke 0.2 μm, nke na-eme ka mgbanwe gas dị irè ma na-egbochi mmetọ nje bacteria na ịbanye mmiri, na-eme ka ọnọdụ uto sel kacha mma.
Nkọwapụta Isi na Ntuziaka Ojiji
- 1. Nhazi olu ọrụnke
- •Ekwesịrị idobe olu dị n'etiti na 20-30% nke ike zuru oke nke karama ahụ iji hụ na nnyefe oxygen zuru oke ma belata nrụgide ịkpụ
- • Akara ngụcha akwụkwọ doro anya dị na karama ahụ na-enye ohere ịlele olu ziri ezi .
- •Ekwesịrị idobe olu dị n'etiti na 20-30% nke ike zuru oke nke karama ahụ iji hụ na nnyefe oxygen zuru oke ma belata nrụgide ịkpụ
- 2. Nhazi nke Na-ama jijijinke
- •Akwadoro yaihe eji agwakọta incubatorọsọ ịma jijiji: 75–120 RPM (enwere ike ịgbanwe dabere na mkpa nnwale)
- •Maka karama ndị buru ibu (≥3L), belata ọsọ ruo 90 RPM iji gbochie oke nkụcha na ụfụfụ mmiri
- •Akwadoro yaihe eji agwakọta incubatorọsọ ịma jijiji: 75–120 RPM (enwere ike ịgbanwe dabere na mkpa nnwale)
Usoro akwadoro maka mkpụrụ ndụ CHO & HEK 293
| Olu nke iko | Olu ọrụ | Ọsọ ịma jijiji |
|---|---|---|
| 125 ml | 25 ml | 120 RPM |
| 250 ml | 60 ml | 120 RPM |
| 500 ml | 150 ml | 120 RPM |
| 1000 ml | 350 ml | 120 RPM |
| 1500 ml | 400 ml | 120 RPM |
| 2000 ml | 400 ml | 120 RPM |
| 3000 ml | 800 ml | 90 RPM |
| 5000 ml | 2500 ml | 90 RPM |
Rịba ama: Data metụtara ahịrị mkpụrụ ndụ CHO Stable, CHO Transient, na HEK 293 Transient
Nkọwa Teknụzụ Mgbakwunye
- 1. Ihe na Nkwarụnke
- • Nhọrọ ihe PETG/PC:PETG na-enye nghọta dị elu na nguzogide mmetụta; PC na-enye nkwụsi ike okpomọkụ dị elu (na-eguzogide autoclaving na 121°C)
-
- •Nkwụsị Ọgwụ: Ejiri gamma mee ka ọ gbaa ọkụ, nke e ji otu n'otu kechie, nke nwere asambodo DNase/RNase enweghị, nke enweghị endotoxin, ma rube isi n'ụkpụrụ USP Class VI
- • Nhọrọ ihe PETG/PC:PETG na-enye nghọta dị elu na nguzogide mmetụta; PC na-enye nkwụsi ike okpomọkụ dị elu (na-eguzogide autoclaving na 121°C)
- 2. Uru Nhazinke
- •Mkpuchi Ergonomic: Na-eme ka njikwa dị nchebe na ndakọrịta na pipettes/cell scrapers dị mfe
- •Ụdị Ndị Na-adịghị Mma Dị: Na-eme ka arụmọrụ ngwakọta dịkwuo mma maka ọdịbendị dị elu site na ịbawanye nnyefe oxygen (kLa)
- •Mkpuchi Ergonomic: Na-eme ka njikwa dị nchebe na ndakọrịta na pipettes/cell scrapers dị mfe
- 3. Ihe Ndị Dị Mkpa Banyere Ojijinke
- •Zere Ịjupụta Oke: Oke karịrị 30% nwere ike ibelata mgbanwe gas, na-eduga na hypoxia na omenala aerobic
- •Lelee pH/Oxygen: Nọgide na-enwe pH na 7.0–7.4 (CHO cells) yana oxygen agbazere agbaze (DO) karịa 40% maka metabolism kacha mma
- •Mmetụta Ịkpụkpụ: Maka mkpụrụ ndụ ndị na-enwe mmetụta ịkpụkpụ (dịka ọmụmaatụ, mkpụrụ ndụ T bụ isi), jiri obere ọsọ (80–100 RPM) ma ọ bụ ihe na-egbochi ụfụfụ
- •Zere Ịjupụta Oke: Oke karịrị 30% nwere ike ibelata mgbanwe gas, na-eduga na hypoxia na omenala aerobic
Ngwa Ndị Karịrị Ọkụkọ Ọkọlọtọ
- •Sistemụ Perfusion: Jikọta ya na ahịrị nri maka inye nri na-aga n'ihu, na-enweta njupụta sel >20 × 10⁶ sel/mL
- •Omenala Microbial: Dabara adaba maka ịgbakọ nje bacteria/yist yana atụmatụ ndị na-adịghị mma maka mma ikuku
- •Usoro Nhazi Elu: Mgbe ị na-agbanwe site na flasks gaa na bioreactors, nọgide na-enwe ọnụego nnyefe oxygen volumetric (kLa) site na ịgbanwe mkpali na ikuku
Oge ozi: Ọgọst-17-2025