Immisa dhexdhexaad ah ayaa lagu darayaa dhalada ku jirta weel lagu ruxayo?
Dhalooyinka Erlenmeyer ee dhaqanka unugyada nadiifka ah ee RADOBIO waxaa si gaar ah loogu talagalay in lagu beero unugyada joojinta oo ay ku jiraan unugyada HEK 293 iyo CHO ee ku jiraQalabka kilinikada ruxayaDaboolkan wuxuu leeyahay salka fidsan iyo dabool hawo leh, taasoo ka dhigaysa mid ku habboon horumarinta geeddi-socodka yar yar, ballaarinta, iyo beerista marxalado badan leh. Daboolka hawo-mareenka leh wuxuu isku daraa xuub hydrophobic ah oo 0.2 μm ah, kaas oo suurtogal ka dhigaya is-weydaarsiga gaaska ee hufan iyadoo la xannibayo wasakhowga microbial iyo gelitaanka biyaha, taasoo hubinaysa xaaladaha koritaanka unugyada ugu wanaagsan.
Tilmaamaha Muhiimka ah iyo Tilmaamaha Isticmaalka
- 1. Hagaajinta Mugga Shaqada;
- •Mugga dhexdhexaadka ah waa in lagu hayaa 20-30% wadarta guud ee awoodda dhalada si loo hubiyo wareejinta oksijiinta ku filan iyo yareynta cadaadiska xiirashada
- •Calaamadaha qalin-jabinta ee cad ee ku yaal dhalada ayaa oggolaanaya cabbir sax ah oo mugga ah .
- •Mugga dhexdhexaadka ah waa in lagu hayaa 20-30% wadarta guud ee awoodda dhalada si loo hubiyo wareejinta oksijiinta ku filan iyo yareynta cadaadiska xiirashada
- 2. Cabbirrada Ruxitaanka;
- •Lagu taliyayqalabka lagu nadiifiyo qalabka lagu nadiifiyoXawaaraha ruxitaanka: 75–120 RPM (waa la hagaajin karaa iyadoo lagu saleynayo baahiyaha tijaabada)
- •Dhalooyinka waaweyn (≥3L), yaree xawaaraha ilaa 90 RPM si looga hortago xiirashada xad-dhaafka ah iyo xumbo.
- •Lagu taliyayqalabka lagu nadiifiyo qalabka lagu nadiifiyoXawaaraha ruxitaanka: 75–120 RPM (waa la hagaajin karaa iyadoo lagu saleynayo baahiyaha tijaabada)
Cabbiraadaha lagu taliyay ee unugyada CHO & HEK 293
| Mugga Buufinta | Mugga Shaqada | Xawaaraha Ruxitaanka |
|---|---|---|
| 125 mL | 25 mL | 120 RPM |
| 250 mL | 60 mL | 120 RPM |
| 500 mL | 150 mL | 120 RPM |
| 1000 mL | 350 mL | 120 RPM |
| 1500 mL | 400 mL | 120 RPM |
| 2000 mL | 400 mL | 120 RPM |
| 3000 mL | 800 mL | 90 RPM |
| 5000 mL | 2500 mL | 90 RPM |
Fiiro gaar ah: Xogtu waxay khuseysaa khadadka unugyada ku meel gaarka ah ee CHO Stable, CHO Transient, iyo HEK 293
Faahfaahinta Farsamada ee Dheeraadka ah
- 1.Qalabka iyo Nadiifnimada;
- • Ikhtiyaarada Agabka PETG/PC:PETG waxay bixisaa hufnaan sare iyo iska caabin saameyn; PC waxay bixisaa xasillooni kuleyl oo heer sare ah (waxay u adkaysataa is-xakamaynta 121°C)
-
- •Jeermis-ka-saarid: Shaabadaysan oo lagu shubay gamma, si gaar ah loo baakadeeyay, DNase/RNase-la'aan ah, aan lahayn endotoxin, oo u hoggaansan heerarka USP Class VI
- • Ikhtiyaarada Agabka PETG/PC:PETG waxay bixisaa hufnaan sare iyo iska caabin saameyn; PC waxay bixisaa xasillooni kuleyl oo heer sare ah (waxay u adkaysataa is-xakamaynta 121°C)
- 2. Faa'iidooyinka Naqshadeynta;
- • Koofiyad Ergonomic ah: Waxay sahlaysaa maaraynta sugan iyo iswaafajinta tuubooyinka/xoqayaasha unugyada
- •Moodooyinka La Khalday ee La Heli Karo: Waxay kor u qaadaysaa hufnaanta isku-darka ee dhaqamada cufnaanta sare leh iyadoo la kordhinayo wareejinta oksijiinta (kLa)
- • Koofiyad Ergonomic ah: Waxay sahlaysaa maaraynta sugan iyo iswaafajinta tuubooyinka/xoqayaasha unugyada
- 3. Qoraallada Isticmaalka ee Muhiimka ah;
- •Iska ilaali Buuxinta xad-dhaafka ah: Mugga ka badan 30% wuxuu yareyn karaa is-weydaarsiga gaaska, taasoo horseedaysa hypoxia dhaqamada aerobic-ka
- •La soco pH/Oksijiin: Ku hay pH 7.0–7.4 (unugyada CHO) iyo oksijiin milma (DO) >40% si aad u hesho dheef-shiid kiimikaadka ugu wanaagsan
- •Xasaasiga Xiirashada: Unugyada xasaasiga u ah xiirashada (tusaale ahaan, unugyada T ee aasaasiga ah), isticmaal xawaare hoose (80-100 RPM) ama wakiilada ka hortagga xumbada.
- •Iska ilaali Buuxinta xad-dhaafka ah: Mugga ka badan 30% wuxuu yareyn karaa is-weydaarsiga gaaska, taasoo horseedaysa hypoxia dhaqamada aerobic-ka
Codsiyada ka baxsan Beerista Caadiga ah
- •Nidaamyada Perfusion: Ku dar khadadka quudinta si aad u hesho nafaqo joogto ah, adigoo gaaraya cufnaanta unugyada >20 × 10⁶ unugyo/mL
- •Dhaqamada Microbial: Ku habboon halsano bakteeriyada/khamiirka oo leh naqshado wareersan si loo hagaajiyo hawo-qaadista
- •Habraacyada Kor-u-qaadista: Markaad ka gudubto dhalooyinka una gudubto kuwa wax ka celiya noolaha, ilaali heerka wareejinta oksijiinta mugga (kLa) oo joogto ah adigoo hagaajinaya kicinta iyo hawo-siinta
Waqtiga boostada: Agoosto-17-2025