Nawa ya kamata a ƙara matsakaici a cikin kwalbar a cikin incubator mai girgiza?
An ƙera flasks ɗin Erlenmeyer na musamman don haɓaka ƙwayoyin dakatarwa waɗanda suka haɗa da ƙwayoyin HEK 293 da CHO a cikin wanigirgiza incubatorWaɗannan kwalaben suna da ƙasa mai faɗi da murfi mai iska, wanda hakan ya sa suka dace da ƙananan matakai na haɓaka tsari, haɓaka girma, da kuma noman matakai da yawa. Murfin da aka hura yana haɗa membrane mai kama da hydrophobic membrane 0.2 μm, wanda ke ba da damar musayar iska mai inganci yayin da yake toshe gurɓatar ƙwayoyin cuta da shigar ruwa cikin ruwa, yana tabbatar da yanayin haɓakar ƙwayoyin halitta mafi kyau.
Muhimman bayanai da jagororin amfani
- 1. Inganta Girman Aiki;
- •Ya kamata a kiyaye matsakaicin girma a 20-30% na jimlar ƙarfin kwalba don tabbatar da isasshen canja wurin iskar oxygen da rage matsin lamba na yankewa
- • Alamomin kammala karatu a kan kwalbar suna ba da damar auna daidai girman ƙara .
- •Ya kamata a kiyaye matsakaicin girma a 20-30% na jimlar ƙarfin kwalba don tabbatar da isasshen canja wurin iskar oxygen da rage matsin lamba na yankewa
- 2. Sigogi Masu Girgizawa;
- •An ba da shawararna'urar girgiza incubatorGudun girgiza: 75-120 RPM (ana iya daidaitawa bisa ga buƙatun gwaji)
- •Ga manyan kwalaben ruwa (≥3L), rage gudu zuwa 90 RPM domin hana fitar ruwa da kumfa mai yawa
- •An ba da shawararna'urar girgiza incubatorGudun girgiza: 75-120 RPM (ana iya daidaitawa bisa ga buƙatun gwaji)
Sigogi da aka ba da shawarar don ƙwayoyin CHO & HEK 293
| Ƙarar walƙiya | Ƙarar aiki | Gudun Girgiza |
|---|---|---|
| 125 ml | 25 ml | 120 RPM |
| 250 ml | 60 ml | 120 RPM |
| 500 ml | 150 ml | 120 RPM |
| 1000 ml | 350 ml | 120 RPM |
| 1500 ml | 400 ml | 120 RPM |
| 2000 ml | 400 ml | 120 RPM |
| 3000 ml | 800 ml | 90 RPM |
| 5000 ml | 2500 ml | 90 RPM |
Lura: Bayanai sun shafi layukan ƙwayoyin CHO Stable, CHO Transient, da HEK 293 Transient cells
Ƙarin Bayanan Fasaha
- 1. Kayan Aiki da Rashin Tsafta;
- • Zaɓuɓɓukan Kayan PETG/PC:PETG yana ba da babban bayyananne da juriya ga tasiri; PC yana ba da kwanciyar hankali mai kyau (yana jure wa autoclaving a 121°C)
-
- •Tsaftacewa: An yi amfani da hasken gamma, an shirya shi daban-daban, an tabbatar da DNase/RNase ba tare da endotoxin ba, kuma ya dace da ƙa'idodin USP Class VI
- • Zaɓuɓɓukan Kayan PETG/PC:PETG yana ba da babban bayyananne da juriya ga tasiri; PC yana ba da kwanciyar hankali mai kyau (yana jure wa autoclaving a 121°C)
- 2. Fa'idodin Zane;
- • Huluna Mai Daidaito: Yana sauƙaƙa sarrafawa da dacewa da pipettes/cell scrapers
- •Samfuran da ba su da tabbas: Yana haɓaka ingancin haɗuwa don al'adu masu yawa ta hanyar ƙara canja wurin iskar oxygen (kLa)
- • Huluna Mai Daidaito: Yana sauƙaƙa sarrafawa da dacewa da pipettes/cell scrapers
- 3. Bayanan Amfani Masu Muhimmanci;
- •Guji Cikewa da Yawa: Girman da ya wuce kashi 30% na iya rage musayar iskar gas, wanda ke haifar da hypoxia a cikin al'adun aerobic
- •Kula da pH/Oxygen: Kula da pH a 7.0–7.4 (Cells CHO) da kuma iskar oxygen da aka narkar (DO) sama da kashi 40% don ingantaccen metabolism
- •Jin Daɗin Ragewa: Don ƙwayoyin da ke da saurin yankewa (misali, ƙwayoyin T na farko), yi amfani da ƙananan gudu (80-100 RPM) ko magungunan hana kumfa
- •Guji Cikewa da Yawa: Girman da ya wuce kashi 30% na iya rage musayar iskar gas, wanda ke haifar da hypoxia a cikin al'adun aerobic
Aikace-aikace Bayan Noma Na yau da kullun
- •Tsarin Perfusion: Haɗa shi da layukan abinci don ci gaba da samar da sinadarai masu gina jiki, cimma yawan ƙwayoyin halitta sama da 20 × 10⁶ ƙwayoyin halitta/mL
- •Al'adun Microbial: Ya dace da fermentation na ƙwayoyin cuta/yist tare da ƙira masu rikitarwa don inganta iskar shaka
- •Tsarin Haɓaka Sikeli: Lokacin da ake canzawa daga flasks zuwa bioreactors, a kula da daidaitaccen saurin canja wurin iskar oxygen (kLa) ta hanyar daidaita tashin hankali da iska
Lokacin Saƙo: Agusta-17-2025