Ni ingano zingana iki zigomba kongerwa mu icupa riri mu cyuma gishyushya amazi?

Ibisobanuro by'ingenzi n'amabwiriza agenga imikoreshereze​

1. 1.​​Kunoza ubwinshi bw'amajwi mu kaziEse?
   •   •Ubunini buringaniye bugomba kuguma kuri 20–30% by'ubushobozi bwose bw'icupa kugira ngo umwuka uhumure neza kandi ugabanye uburemere bwo gukata
      
   • •Amanota y'irangiza ku icupa asobanutse neza atuma umuntu apima neza ingano y'amazi
      .
2. 2.​Ibipimo byo KunyeganyezaEse?
   • •Bisabweicyuma gishyushya incubatorumuvuduko wo gutigita: ​​75–120 RPM​ (ishobora guhindurwa hashingiwe ku byo igerageza rikeneye)
      
   • •Ku macupa manini (≥3L), gabanya umuvuduko kugeza kuri 90 RPM​ kugira ngo wirinde gukata amazi menshi no gufuro

---

Ibipimo byasabwe kuri CHO na HEK 293 Seli

Icyitonderwa: Amakuru areba imirongo ya selile ya CHO Stable, CHO Transient, na HEK 293 Transient

---

Ibisobanuro by'inyongera bya tekiniki​

1. 1.​Ibikoresho n'UbutavuroEse?
   • •Amahitamo y'ibikoresho bya PETG/PC​:PETG itanga ubwisanzure kandi irwanya ingaruka; PC itanga ubushyuhe buhamye (ihangana no kwiyatsa kuri 121°C)
     •  
   • •Gusukura: Bikozwe mu buryo bwa gamma, bipfunyitse ukwabyo, nta DNase/RNase yemewe, nta endotoxin irimo, kandi byujuje ibisabwa na USP Class VI
      
2. 2. Ibyiza by'igishushanyoEse?
   • •​​Umugozi w’ingufu​​: Yorohereza imicungire myiza no guhuza neza na pipettes/cell scrapers
      
   • •Uburyo bwa Baffled buhari: Bwongera ubushobozi bwo kuvanga imiterere y’ibimera bifite ubucucike bwinshi binyuze mu kongera uburyo bwo kohereza ogisijeni (kLa)
      
3. 3.​Inyandiko z'ingenzi ku ikoreshwaEse?
   • •Irinde kuzuza byinshi: Ubwinshi >30% bushobora kugabanya ihindagurika ry'imyuka, bigatera hypoxia mu mikurire y'umubiri.
      
   • •Kugenzura pH/Ogisijeni: Gumana pH kuri 7.0–7.4 (uturemangingo twa CHO) na ogisijeni ishongeshejwe (DO) >40% kugira ngo habeho uburyo bwiza bwo gutunganya imikorere y'umubiri
      
   • •Uburyo bwo Gupima Gukata: Ku tunyangingo twibasirwa no gukata (urugero, utwuma twa T tw’ibanze), koresha umuvuduko muto (80–100 RPM) cyangwa imiti irwanya ifuro

---

Ikoreshwa rirenze ikoreshwa ry’ubuhinzi busanzwe

• •Sisitemu zo Gutera Amavuta: Huzwa n'imirongo y'ibiryo kugira ngo intungamubiri zikomeze kuboneka, ugere ku bucucike bw'uturemangingo >20 × 10⁶ uturemangingo / mL
   
• •Udukoko twa mikorobe: Bikwiriye gushyushya bagiteri/umusemburo hamwe n'imiterere idasobanutse kugira ngo umwuka urusheho kumera neza
   
• •Amabwiriza yo Kuzamura: Mu gihe uva mu macupa ujya mu macupa ya bioreactor, komeza umuvuduko uhoraho wo kohereza umwuka wa ogisijeni (kLa) uhindura uburyo bwo guhumeka no guhumeka.