Ingani nzira yekuwedzera inofanira kuwedzerwa muflask iri mu shaken incubator?
Maflask eErlenmeyer eRADOBIO akagadzirwa zvakananga kuti ashandiswe mukurima masero ekudzivirira anosanganisira HEK 293 neCHO masero mune imwe nzvimbo.chinotonhodza chinovhundutsaMaflask aya ane pasi rakati sandara uye chivharo chine mhepo, zvichiita kuti akwanise kugadzirwa muzvikamu zvidiki, kukura, uye kurimwa muzvikamu zvakawanda. Chivharo chine mhepo chinobatanidza 0.2 μm hydrophobic membrane, iyo inobvumira kuchinjana kwegasi zvinobudirira uku ichidzivirira kusvibiswa kwehutachiona uye kupinda kwemvura, zvichiita kuti masero akure zvakanaka.
Magadzirirwo Akakosha uye Nhungamiro dzeMashandisirwo
- 1. Kugadzirisa Vhoriyamu Yekushanda.
- •Vhoriyamu yepakati inofanira kuchengetedzwa pa 20–30% yehuwandu hwese hwechiputiro kuti ive nechokwadi chekufambiswa kweokisijeni kwakakwana uye kuderedza kushushikana kwekucheka.
- •Mavanga ekupedzisira akajeka pafurasiki anoita kuti pave nekuyera vhoriyamu kwakanyatsojeka .
- •Vhoriyamu yepakati inofanira kuchengetedzwa pa 20–30% yehuwandu hwese hwechiputiro kuti ive nechokwadi chekufambiswa kweokisijeni kwakakwana uye kuderedza kushushikana kwekucheka.
- 2.Zviratidzo Zvinozunguza.
- •Zvinokurudzirwachinonyungudutsa chinogara vanakumhanya kwekuzunungusa: 75–120 RPM (inogadziriswa zvichienderana nezvinodiwa zvekuedza)
- •Kana paine maflasks makuru (≥3L), deredza kumhanya kusvika pa90 RPM kudzivirira kuchekwa kwemvura yakawanda uye furo.
- •Zvinokurudzirwachinonyungudutsa chinogara vanakumhanya kwekuzunungusa: 75–120 RPM (inogadziriswa zvichienderana nezvinodiwa zvekuedza)
MaParameter Anokurudzirwa eCHO & HEK 293 Cells
| Vhoriyamu yeFlashi | Vhoriyamu Yekushanda | Kumhanya Kwekuzunungusa |
|---|---|---|
| 125 mL | 25 mL | 120 RPM |
| 250 mL | 60 mL | 120 RPM |
| 500 mL | 150 mL | 120 RPM |
| 1000 mL | 350 mL | 120 RPM |
| 1500 mL | 400 mL | 120 RPM |
| 2000 mL | 400 mL | 120 RPM |
| 3000 mL | 800 mL | 90 RPM |
| 5000 mL | 2500 mL | 90 RPM |
Cherechedza: Data rinoshanda kune CHO Stable, CHO Transient, uye HEK 293 Transient cell lines
Mashoko Ekuwedzera Ehunyanzvi
- 1.Zvinhu uye Kusabereka.
- •Sarudzo dzezvinhu zvePETG/PC:PETG inopa kujeka kwakanyanya uye kuramba kukanganiswa; PC inopa kugadzikana kwakanyanya kwekupisa (inotsungirira autoclaving pa121°C)
-
- •Kuchenesa: Yakapiswa negamma, yakaputirwa yega yega, isina DNase/RNase yakanyoreswa, haina endotoxin, uye inoenderana neUSP Class VI.
- •Sarudzo dzezvinhu zvePETG/PC:PETG inopa kujeka kwakanyanya uye kuramba kukanganiswa; PC inopa kugadzikana kwakanyanya kwekupisa (inotsungirira autoclaving pa121°C)
- 2. Zvakanakira Kugadzira.
- •Ergonomic Cap: Inobatsira kubata zvakanaka uye kushandira pamwe nemapipettes/cell scrapers
- •MaModheru Ane Baffled Anowanikwa: Anowedzera kushanda zvakanaka kwekusanganisa zvirimwa zvine huwandu hwakawanda nekuwedzera kutapurirana kweokisijeni (kLa)
- •Ergonomic Cap: Inobatsira kubata zvakanaka uye kushandira pamwe nemapipettes/cell scrapers
- 3.Mashoko Ekushandisa Akakosha.
- •Dzivisa Kuzadza Zvakawandisa: Huwandu >30% hunogona kuderedza kuchinjana kwegasi, zvichikonzera kushaya mweya mumuviri (hypoxia) mukurovedza muviri.
- •Tarisa pH/Oxygen: Chengetedza pH pa 7.0–7.4 (masero eCHO) uye okisijeni yakanyungudutswa (DO) >40% kuti muviri ushande zvakanaka
- •Kunzwa Kwekucheka: Kune masero anonzwa kutemwa neganda (semuenzaniso, masero ekutanga eT), shandisa kumhanya kwakaderera (80–100 RPM) kana mishonga inorwisa furo
- •Dzivisa Kuzadza Zvakawandisa: Huwandu >30% hunogona kuderedza kuchinjana kwegasi, zvichikonzera kushaya mweya mumuviri (hypoxia) mukurovedza muviri.
Mashandisirwo Anopfuura Kukura Kwemazuva Ano
- •Masisitimu ePerfusion: Shandisa nzira dzekudya kuti muviri urambe uchiwana zvinovaka muviri, zvichiita kuti masero arambe akawandisa >20 × 10⁶ masero/mL
- •Kukura kwezvipembenene: Zvakakodzera kuvirisa bhakitiriya/mbiriso nemagadzirirwo asina kujeka ekuvandudza mhepo
- •Maitiro Ekuwedzera: Paunenge uchichinja kubva kumaflasks kuenda kuma bioreactors, chengetedza chiyero chekufambisa okisijeni (kLa) chinogara chichienderana nekugadzirisa kusimuka uye kufefetera.
Nguva yekutumira: Nyamavhuvhu-17-2025