Kodi ndi zinthu zingati zomwe ziyenera kuwonjezeredwa mu botolo mu chosungiramo zinthu chomwe chikugwedezeka?
Ma flask a RADOBIO sterile cell culture Erlenmeyer amapangidwira makamaka kulima maselo osungunuka kuphatikiza maselo a HEK 293 ndi CHO muchoyatsira chogwedezaMabotolo awa ali ndi pansi lathyathyathya ndi chivundikiro chotulutsa mpweya, zomwe zimapangitsa kuti zikhale zoyenera kupangidwa pang'onopang'ono, kukulitsa, komanso kulima magawo ambiri. Chivundikiro chotulutsa mpweya chimaphatikiza nembanemba ya 0.2 μm hydrophobic, yomwe imalola kusinthana kwa mpweya bwino komanso kuletsa kuipitsidwa kwa tizilombo toyambitsa matenda komanso kulowa kwa madzi, ndikuwonetsetsa kuti maselo akula bwino.
Mafotokozedwe Ofunika ndi Malangizo Ogwiritsira Ntchito
- 1.Kukonza Volume Yogwira Ntchitopa
- •Kuchuluka kwapakati kuyenera kusungidwa pa 20–30% ya mphamvu yonse ya botolo kuti zitsimikizire kuti mpweya umasamutsidwa mokwanira ndikuchepetsa kupsinjika kwa kumeta.
- • Zizindikiro zoonekera bwino pa botolo zimathandiza kuyeza kuchuluka kwa voliyumu molondola. .
- •Kuchuluka kwapakati kuyenera kusungidwa pa 20–30% ya mphamvu yonse ya botolo kuti zitsimikizire kuti mpweya umasamutsidwa mokwanira ndikuchepetsa kupsinjika kwa kumeta.
- 2.Magawo Ogwedezapa
- •Zolangizidwachogwirira choyatsiraliwiro logwedeza: 75–120 RPM (yosinthika kutengera zosowa zoyesera)
- •Pa ma flask akuluakulu (≥3L), chepetsani liwiro kufika pa 90 RPM kuti mupewe kudulidwa kwa madzi ambiri ndi thovu.
- •Zolangizidwachogwirira choyatsiraliwiro logwedeza: 75–120 RPM (yosinthika kutengera zosowa zoyesera)
Ma Parameter Ovomerezeka a CHO & HEK 293 Cells
| Voliyumu ya Botolo | Volume Yogwira Ntchito | Kuthamanga kwa Kugwedeza |
|---|---|---|
| 125 mL | 25 mL | 120 RPM |
| 250 mL | 60 mL | 120 RPM |
| 500 mL | 150 mL | 120 RPM |
| 1000 mL | 350 mL | 120 RPM |
| 1500 mL | 400 mL | 120 RPM |
| 2000 mL | 400 mL | 120 RPM |
| 3000 mL | 800 mL | 90 RPM |
| 5000 mL | 2500 mL | 90 RPM |
Chidziwitso: Deta imagwira ntchito pa CHO Stable, CHO Transient, ndi HEK 293 Transient cell lines
Zambiri Zowonjezera Zaukadaulo
- 1.Zinthu ndi Kusaberekapa
- •Zosankha za Zinthu za PETG/PC:PETG imapereka mawonekedwe owonekera bwino komanso kukana kukhudza; PC imapereka kukhazikika kwabwino kwa kutentha (imapirira autoclaving pa 121°C)
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- •Kuyeretsa: Yopakidwa ndi gamma, yopakidwa payokha, yopanda DNase/RNase yovomerezeka, yopanda endotoxin, komanso yogwirizana ndi miyezo ya USP Class VI
- •Zosankha za Zinthu za PETG/PC:PETG imapereka mawonekedwe owonekera bwino komanso kukana kukhudza; PC imapereka kukhazikika kwabwino kwa kutentha (imapirira autoclaving pa 121°C)
- 2. Ubwino wa Kapangidwepa
- •Ergonomic Cap: Imathandizira kugwirira ntchito bwino komanso kugwirizana ndi ma pipettes/ma cell scrapers
- •Ma Model Osokonezeka Akupezeka: Amawonjezera mphamvu yosakaniza zinthu zomwe zimakhala ndi mpweya wambiri mwa kuwonjezera kusamutsa mpweya (kLa)
- •Ergonomic Cap: Imathandizira kugwirira ntchito bwino komanso kugwirizana ndi ma pipettes/ma cell scrapers
- 3.Zolemba Zofunika Kwambiri Zokhudza Kugwiritsa Ntchitopa
- •Pewani Kudzaza Mopitirira Muyeso: Kuchuluka kwa mpweya >30% kungachepetse kusinthana kwa mpweya, zomwe zimapangitsa kuti pakhale hypoxia m'ma aerobic cultures
- •Yang'anirani pH/Oxygen: Sungani pH pa 7.0–7.4 (maselo a CHO) ndi mpweya wosungunuka (DO) >40% kuti kagayidwe kabwino ka thupi kagwire bwino ntchito
- •Kuzindikira Kudula: Pa maselo omwe amakhudzidwa ndi kudula (monga maselo a T oyambira), gwiritsani ntchito liwiro lotsika (80–100 RPM) kapena mankhwala oletsa thovu
- •Pewani Kudzaza Mopitirira Muyeso: Kuchuluka kwa mpweya >30% kungachepetse kusinthana kwa mpweya, zomwe zimapangitsa kuti pakhale hypoxia m'ma aerobic cultures
Ntchito Zoposa Kulima Koyenera
- •Machitidwe Osakaniza Mafuta: Lumikizani ndi mizere yodyetsera kuti mupeze michere mosalekeza, kuti mupeze kuchulukana kwa maselo >20 × 10⁶ maselo/mL
- •Kumera kwa Tizilombo Tosaoneka ndi Mabakiteriya: Koyenera kuwiritsa mabakiteriya/yisiti ndi mapangidwe osokonezeka kuti mpweya ukhale wabwino
- •Ma Protocol Okulitsa Mphamvu: Mukasintha kuchoka ku ma flask kupita ku bioreactors, sungani kuchuluka kofanana kwa mpweya woipa (kLa) mwa kusintha kugwedezeka ndi mpweya.
Nthawi yotumizira: Ogasiti-17-2025